Lysis buffer Tris-HCl

In the field of cell biology and biochemistry research, maintaining a suitable pH value is conducive to cell lysis. Because Tris-HCl has excellent buffering capacity and is often used as the main component of lysis buffer, various biochemical reactions and the stability of substances in cells are extremely sensitive to pH changes. For example, the activity of many enzymes can only be maintained within a specific pH range, and the structure and function of biological macromolecules such as proteins and nucleic acids in cells also rely on a stable pH environment. Tris-HCl can effectively resist pH fluctuations caused by external factors within a certain range and maintain the pH value of the lysis buffer at an optimal state. This provides a stable chemical environment for the subsequent processing and analysis of intracellular substances.