Blog.

In the field of cell biology and biochemistry research, maintaining a suitable pH value is conducive to cell lysis. Because Tris-HCl has excellent buffering capacity and is often used as the main component of lysis buffer, various biochemical reactions and the stability of substances in cells are extremely sensitive to pH changes. For example, the activity of many enzymes can only be maintained within a specific pH range, and the structure and function of biological macromolecules such as proteins and nucleic acids in cells also rely on a stable pH environment. Tris-HCl can effectively resist pH fluctuations caused by external factors within a certain range and maintain the pH value of the lysis buffer at an optimal state. This provides a stable chemical environment for the subsequent processing and analysis of intracellular substances.

In the field of chemiluminescence detection, luminol, as an important chemiluminescence reagent, has attracted much attention due to its unique luminescence properties. There are various methods for luminol detection. The following three methods are commonly used. Let’s take a look.

In the fields of chemical experiments and biological research, it is often necessary to prepare specific chemical substances into solutions to meet various experimental needs. MES sodium salt is a commonly used chemical reagent. It is a derivative of MES buffer and its Chinese name is 2-morpholineethanesulfonic acid sodium salt. Its original property is white powder. When used, it needs to be configured into a solution. How to configure it? Let's take a look.

During cell culture, it is important to maintain a stable pH environment. HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer is widely used in cell culture systems due to its good buffering properties and biocompatibility. Since cell growth and metabolism are very sensitive to pH, small changes in pH may affect cell physiological functions, enzyme activity, and protein synthesis and folding. HEPES buffer can resist the influence of external acids and bases within a certain range and keep the pH of the cell culture environment relatively stable. So how to prepare HEPES buffer solution? Let's take a look.

In the field of chemiluminescent analysis, acridinium ester, as an important chemiluminescent marker, plays a key role in many fields with its unique properties and advantages. Acridinium ester appears as a yellow powder. It has high chemical stability and is easy to store, which provides convenience for its practical application. According to the different substituents, acridinium substituents commonly used as chemiluminescent markers are divided into two categories: acridinium esters and acridinium sulfonamides. They all have a common acridinium ring in their structures, and this structural feature determines their similar luminescence mechanism.